Breeding with DNA
We use double digested restriction associated DNA genotyping (ddRADseq) as it is a cost-effective method that allow to sequence a reduced representation of the genome, still producing thousands of markers per sample. It is flexible and allow to work both with species with and without a reference genome.
Advantage of our technology:
– High marker yield (>5’000 SNPs/sample)
– Cost effective
– No reference genome needed
– Various post-hoc analysis possible
Traditionally, animal breeding and inbreeding estimation have relied on pedigree data from stud books. With the development of genetic, pedigrees have been inferred genetically. NGS have shifted the way we calculate inbreeding. We do not infer pedigree genetically, but we compute inbreeding at the genomic level. The high marker yield allows our bioinformatics pipelines to produce reliable and precise inbreeding and kinship estimates that have been proven to be linked with health related traits. Beyond this, the data can be exploited further to describe population structures, to map quantitative trait loci or to determine genetic sex.
– DNA extraction and normalization
– ddRADseq library preparation
– Illumina sequencing
– Bioinformatic analysis
– Data reporting & breeding scheme